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In vitroantioxidant of the protein hydrolysate isolated from the seeds of hoary basil (Ocimum basilicum) |
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| รหัสดีโอไอ | |
| Creator | Karittha Semanit, Jittra Piapukiew, Sajee Noitang and AphichartKarnchanatat |
| Title | In vitroantioxidant of the protein hydrolysate isolated from the seeds of hoary basil (Ocimum basilicum) |
| Publisher | Faculty of Agro-Industry |
| Publication Year | 2558 |
| Journal Title | Food and Applied Bioscience |
| Journal Vol. | 3 |
| Journal No. | 2 |
| Page no. | 150-159 |
| Keyword | hoary basil, protein hydrolysate, antioxidant activity |
| URL Website | www.tci-thaijo.org/index.php/fabjournal |
| Website title | www.tci-thaijo.org/index.php/fabjournal/article/view/78188 |
| ISSN | 2286-8615 |
| Abstract | Oxidation plays an important role in the basic processes of life, such as the production of energy and phagocytises employed by the immune system. However, when an imbalance between oxidants and antioxidants exists in vivo, oxidation can become uncontrolled and result in diseases such as arthritis, cancer, atherosclerosis, and Alzheimer's disease. The objective of this research work to produce hoary basil seedprotein hydrolysates (HBPs) and their peptides with antioxidant properties. Enzymatic hydrolysis of the seeds was performed by using a bacterial alkaline protease, Protease G6 at their optimal conditions. The degree of hydrolysis of HBPs with Protease G6 hydrolysis and DPPH radical scavenging assay, IC50values showed 55.134?0.10% and 0.0196?0.064 ?g/mL for 270 min. These results show that HBPs increased the degree of hydrolysis and antioxidant activity of protein hydrolysates. HBPs were fractionated into three fractions, HBPs-I (MW> 10 kDa), HBPs-II (MW = 510 kDa), and HBPs-III (MW < 5 kDa) and the in vitroantioxidant activities of all fractions were determined. HBPs-III showed the lowest IC50value at 0.02523?0.14 ?g/mL for DPPHradical scavenging activity.The findings suggestthe importance of hoary basil seedprotein as a source of bioactive peptides. |
