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Development of Tetra-primer ARMS-PCR Technique for High Amylose Selection in Cassava Breeding |
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| รหัสดีโอไอ | |
| Creator | Adcharapun Chaicharoen |
| Title | Development of Tetra-primer ARMS-PCR Technique for High Amylose Selection in Cassava Breeding |
| Contributor | Suwaluk Sansanee, Jeeraporn Kansup, Vipavee Chanroj, Krittaya Petchpoung, Siriwan Soiklom |
| Publisher | Department of Agriculture |
| Publication Year | 2569 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 44 |
| Journal No. | 2 |
| Page no. | 191-201 |
| Keyword | amylose content, molecular marker, SNP, Tetra-primer ARMS-PCR, plant breeding |
| URL Website | tci-thaijo.org/index.php/thaiagriculturalresearch |
| Website title | Thai Agricultural Research Journal |
| ISSN | 3027-7272 |
| Abstract | Amylose starch in cassava (Manihot esculenta Crantz) can be widely used in many industries. Rapid selection of cassava varieties with high amylose starch content can be done using advanced techniques. This study aimed to develop molecular markers associated with high amylose content by designing primers for single nucleotide polymorphism (SNP) markers. The results revealed that the SNP marker S2_25293441 on chromosome 2 was significantly associated with high amylose content. Optimal conditions for tetra-primer amplification refractory mutation system polymerase chain reaction (tetra-primer ARMS-PCR) were achieved at an annealing temperature of 60?C. In heterozygous (CT) genotypes, DNA bands of 339, 229, and 166 bp were observed, while homozygous (CC) genotypes produced bands of 339 and 229 bp. Genotypes exhibiting the CT genotype demonstrated phenotypes with high amylose content. In testing with cassava varieties containing amylose content exceeding 18%, the developed SNP marker achieved an accuracy rate of 75.90% in selecting high amylose varieties. The use of this molecular marker reduced the number of plants required for field selection, enabled screening with fairly high accuracy, and shortened the time for selecting cassava varieties with high amylose content. |
