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Effects of zeatin and gibberellic acid on regeneration and in vitro flowering of Phlox paniculata L. |
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| รหัสดีโอไอ | |
| Creator | Nordiyanah Anuar |
| Title | Effects of zeatin and gibberellic acid on regeneration and in vitro flowering of Phlox paniculata L. |
| Contributor | Noraini Mahmad, Sharifah Nurashikin Wafa, Sakinah Abdullah, Hashimah Elias |
| Publisher | Maejo University |
| Publication Year | 2560 |
| Journal Title | Maejo International Journal of Science and Technology |
| Journal Vol. | 11 |
| Journal No. | 2 |
| Page no. | 115 |
| Keyword | Phlox paniculata L., in vitro flowering, zeatin, gibberellic acid, micropropagation |
| Website title | Maejo International Journal of Science and Technology |
| ISSN | 1905-7873 |
| Abstract | The present work reports on the regeneration and in vitro flowering of Phlox paniculata L. Different explants (leaves, petioles and stems) were cultured on Murashige and Skoog (MS) solid medium supplemented with different concentrations and combinations of -napthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP). Among the explants studied, leaves were found to be the most responsive and had the highest regeneration frequency (100%) when they were cultured in the presence of 8.0 uM NAA and 6.6 uM BAP. However, the regenerated plantlets did not produce any flower buds in successive subcultures. In vitro flowering was only induced after 4 weeks of culturing from shoot tip segments which were maintained on MS solid medium supplemented with zeatin (2.2-11.2 uM) or gibberellic acid (GA3,1.4-7.2 uM). In the present study treatment with 11.2 uM zeatin showed the highest frequency of in vitro flowering (67.8 3.2%), whereas 5.6 uM GA3 gave a lower percentage (48.8 9.3%). Although the flowers generated in vitro were sterile, our study would provide an important step towards future investigation on the essential factors in in vitro flowering in P. paniculata and to elucidate other developmental, physiological and environmental stimuli, which are required for promoting or inhibiting the transition of a vegetative state to a flowering state in this species. |
