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Polymorphism and association study of candidate genes on Salmonella fecal shedding in pigs |
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| รหัสดีโอไอ | |
| Creator | Pitchayanipa Phongphanich |
| Title | Polymorphism and association study of candidate genes on Salmonella fecal shedding in pigs |
| Contributor | Nattariga Suwannawong, Uschara Thumarat, Thunchira Thepparat |
| Publisher | Faculty of Agriculture |
| Publication Year | 2568 |
| Journal Title | Khon Kaen Agriculture Journal |
| Journal Vol. | 53 |
| Journal No. | 3 |
| Page no. | 542-558 |
| Keyword | CCT7, salmonella, diarrhea, pig |
| URL Website | https://li01.tci-thaijo.org/index.php/agkasetkaj |
| Website title | Khon Kaen Agriculture Journal |
| ISSN | 3027-6497 (Online) |
| Abstract | The objective of this study was to investigate the genetic polymorphism in four candidate genes, consisting of: Chaperonin Containing TCP1 Subunit 7 (CCT7) gene, Alpha-1-Fucosyltransferase (FUT1) gene, Neutrophil Cytosolic Factor 2 (NCF2) gene and Toll-like receptors 4 (TLR4) gene and their association with fecal score and salmonella fecal shedding in pigs. In this study, 40 crossbred pigs (50% Duroc x 25% Large White x 25% Landrace) at 15-21 days of age were oral inoculated with Salmonella (Salmonella Typhimurium) and recorded for fecal scores and salmonella fecal shedding on 2, 7, 14- and 21-days post inoculation (dpi). The polymorphism of four candidate genes were genotyped using PCR-RFLP method. Analysis of the association between functional candidate genes and diarrhea scores using Cochran-Mantel-Haenszel test and Salmonella shedding levels using General Linear Model procedure (GLM). The result showed that there was no statistical difference in the fecal scores of pigs after day post inoculation (P>0.05). The amount of Salmonella shedding after day post inoculation was statistically different. (P<0.05) which pigs had the highest level of shedding of Salmonella on the 7 dpi. The polymorphism of CCT7 gene showed three genotypic variations and FUT1 gene showed two genotypic variations. Meanwhile, the NCF2 gene and TLR4 gene had monomorphic. Association analysis showed that no significant between fecal score and genotype pattern of CCT7 and FUT1 but the polymorphisms of CCT7 gene significant associations with salmonella fecal shedding. Genotype AA had highest salmonella fecal shedding at 2 dpi (3.05 x104 CFU/ml). Therefore, CCT7 genes may be used as a candidate marker gene for genetic improvement of salmonella infection resistance in pigs. |
