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Co-Exposure to Particulate Matter and Ultraviolet B Radiation Induces Keratinocyte Apoptosis and Impairs Wound Healing In Vitro |
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| รหัสดีโอไอ | |
| Creator | Anyamanee Chatsirisupachai |
| Title | Co-Exposure to Particulate Matter and Ultraviolet B Radiation Induces Keratinocyte Apoptosis and Impairs Wound Healing In Vitro |
| Contributor | Nontaphat Leerach, Praepilai Piriyapratankun, Peerawut Cheunmatcha, Jaturon Kwanthongdee, Pawin Pongkorpsakol, Anyamanee Chatsirisupachai |
| Publisher | Thai Society of Higher Education Institutes on Environment |
| Publication Year | 2569 |
| Journal Title | EnvironmentAsia |
| Journal Vol. | 19 |
| Journal No. | 1 |
| Page no. | 32-43 |
| Keyword | Ultraviolet B, Particulate matter, Keratinocytes, Wound healing |
| URL Website | http://www.tshe.org/ea/index.html |
| Website title | EnvironmentAsia |
| ISSN | 1906-1714 |
| Abstract | Environmental stressors such as ultraviolet B (UVB) radiation and airborne particulate matter (PM) are known to damage skin; however, their combined effects on epidermal responses remain poorly understood. This study evaluated the impact of co-exposure to UVB and PM on apoptosis and wound healing in HaCaT human keratinocytes in vitro. To mimic real-world conditions, both acute (30 minutes–24 hours) and chronic (>24 hours) exposures were assessed. Cells were treated with UVB (25–200 mJ/cm²) and PM (12.5–200 µg/mL), individually or in combination. Apoptotic activity was analyzed using flow cytometry with a caspase-3/7 probe, while wound healing and migration were examined by scratch assay. Cell morphology and nuclear integrity were further evaluated by Giemsa staining. Results showed that co-exposure disrupted membrane and nuclear organization, reduced cell migration, and delayed wound closure compared to single exposures. Caspase-3/7 activity was significantly elevated, particularly at PM 50 µg/mL with UVB 200 mJ/cm² (p < 0.0001). Chronic co-exposure also increased apoptotic cell populations, indicating persistent stress and cumulative damage. These findings suggest that combined UVB and PM exposure aggravates skin injury by enhancing apoptosis and impairing repair. The study underscores the toxicological significance of environmental co-exposures and highlights the need for protective and therapeutic strategies. |
