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Optimization of γ-aminobutyric acid production by Lactiplantibacillus plantarum isolated from Vietnamese fermented pork (nem chua) using central composite design |
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| รหัสดีโอไอ | |
| Creator | Huynh X Phong |
| Title | Optimization of γ-aminobutyric acid production by Lactiplantibacillus plantarum isolated from Vietnamese fermented pork (nem chua) using central composite design |
| Contributor | Nguyen N Thanh, Tran H Duyen, Nguyen N H Binh, Luu M Chau, Le Q Viet, Bui H D Long, Warayutt Pilap, Sudarat Thanonkeo, Pornthap Thanonkeo |
| Publisher | Khon Kaen University, Thailand |
| Publication Year | 2569 |
| Journal Title | Asia-Pacific Journal of Science and Technology |
| Journal Vol. | 31 |
| Journal No. | 1 |
| Page no. | 15 (10 pages) |
| Keyword | γ-aminobutyric acid, Central Composite Design, Fermented pork, Lactiplantibacillus plantarum, Nem chua |
| URL Website | https://apst.kku.ac.th/ |
| Website title | https://apst.kku.ac.th/optimization-of-%ce%b3-aminobutyric-acid-production-by-lactiplantibacillus-plantarum-isolated-from-vietnamese-fermented-pork-nem-chua-using-central-composite-design/ |
| ISSN | 2539-6293 |
| Abstract | Lactic acid bacteria (LAB) capable of biosynthesizing gamma-aminobutyric acid (GABA) have attracted increasing interest due to their status as safe microorganisms in food preservation and processing. In this study, GABA content was quantified using thin-layer chromatography (TLC), and optimal fermentation conditions, including initial concentrations of monosodium glutamate (MSG), Tween 80, and pH, were modeled using response surface methodology (RSM). The results indicated that Lactiplantibacillus plantarum can produce GABA at a concentration of 1.533 mg/mL after 72 h of fermentation at 37ºC in de Man, Rogosa, and Sharpe (MRS) broth supplemented with 1% MSG at pH 5.0. RSM analysis using central composite design (CCD) demonstrated that the maximum GABA production of 1.911 mg/mL was achieved in an optimized MRS medium with an initial pH of 4.97, supplemented with 59.72 g/L of MSG and 2.05 g/L of Tween 80. |
