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Ethyl methane sulfonate (EMS) enhanced the formation of leaf glandular trichomes and the production of artemisinin in Artemisia annua L. |
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| รหัสดีโอไอ | |
| Creator | E-Shuen Leow |
| Title | Ethyl methane sulfonate (EMS) enhanced the formation of leaf glandular trichomes and the production of artemisinin in Artemisia annua L. |
| Contributor | E-Shuen Leow, Nadali B. Jelodar, Lai-Keng Chan |
| Publisher | Asia-Pacific Journal of Science and Technology |
| Publication Year | 2563 |
| Journal Title | Asia-Pacific Journal of Science and Technology |
| Journal Vol. | 25 |
| Journal No. | 1 |
| Page no. | 1-9 |
| Keyword | Artemisia annua L., Artemisinin, Ethyl methane sulfonate, Glandular trichome, In vitro plantlets |
| URL Website | https://www.tci-thaijo.org/index.php/APST |
| Website title | https://so01.tci-thaijo.org/index.php/APST/article/view/188463/163690 |
| ISSN | 2539-6293 |
| Abstract | Artemisia annua L.can naturally synthesize artemisinin, an anti-malarial compound. Due to the naturally low content of artemisinin available in this plant, many different approaches were used to increase the production of artemisinin. The objective of our study was to determine whether ethyl methane sulfonate (EMS) treated seeds could enhance the formation of leaf glandular trichomes and increase the artemisinin content in the plants. The in vitro plantlets were established from EMS treated and untreated seeds on a basic Murashige and Skoog solid medium. The morphology of the leaf glandular trichomes was observed under a fluorescence microscope (Olympus FX53) at the blue wavelength (460-495 nm) while the artemisinin content was determined using High-performance liquid chromatography (HPLC). Results indicated that EMS did not affect the plantlet growth but caused temporary morphological abnormality in < 17% of the in vitro plantlets in the form of leaf albinism and chlorosis which disappeared after four subculture cycles. EMS of 0.5-1% (v/v) used for the seed treatment was able to induce the formation of more leaf glandular trichomes for all the three tested A. annua L. clones. While EMS ranging between 1.0% (v/v) and 2.0% (v/v) caused an increase in trichome size. A higher artemisinin content was detected in plantlets derived from seeds treated with 1.0% (v/v) EMS. Hence it was concluded that 1% (v/v) EMS could be used to induce the formation of more and bigger leaf trichomes that enhance the production of artemisinin in A. annua L. |
