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Roselle(Hibiscussabdariffa) calyce ethanolic extract decreases insulin resistance in high carbohydrate diet fed cats |
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| รหัสดีโอไอ | |
| Creator | Ranee Singh |
| Title | Roselle(Hibiscussabdariffa) calyce ethanolic extract decreases insulin resistance in high carbohydrate diet fed cats |
| Contributor | Patchareewan Pannangpetch |
| Publisher | Research and Technology Transfer Affairs Division.Khon Kaen University. |
| Publication Year | 2560 |
| Journal Title | Asia-Pacific Journal of Science and Technology (APST) |
| Journal Vol. | 22 |
| Journal No. | 4 |
| Page no. | 1-7 |
| Keyword | Roselle, Triglyceride, Cholesterol, Blood glucose, Insulin resistance |
| URL Website | https://tci-thaijo.org/index.php/APST/index |
| Website title | https://tci-thaijo.org/index.php/APST/article/view/107648 |
| ISSN | 2539-6293 |
| Abstract | The objective of this study was todetermine whether roselle calyce extract couldmodulate insulin resistance inhigh carbohydrate diet fed cats.Fifteen non-obese, mixed-breed, adult clinically healthy cats were used in this study. Following consumption of a commercially available control diet for 2 weeks, venous blood wascollected to determine the baseline of blood glucose. Then the cats were randomly separated into 5 groups of 3 cats each. Group 1 received the control diet throughout the experiment. Groups 2-5 received high carbohydrate diets(HC) for 2 weeks to induce insulin resistance. After that, group 2 continued to receive a HC diet for further 5 weeks while groups 3 and 4 received a HC diet with roselle extract at doses of 0.1 or 1g/kg BW/day and group 5 received acarbose 25 mg/cat orally once daily. An intravenous glucose tolerance test (IVGTT) was performed after the 4thweek of treatments in all groups andfasting blood were collected to measure insulin, glucose, triglycerides, cholesterol, andBUN. The fasting glucose and insulin values were used to calculate Homeostasis model assessment for insulin resistance (HOMA-IR). Then in the 5thweek,blood samples were collected over 24 hours at 0min, 30 min, 1, 2, 3, 4, 6, 8, 11, 14, 18and 24 hr for monitoring of 24 hoursglucose. The IVGTT showed the area under the plasma glucosetime curve (AUC)of HCdiet control group was significantly higher than that of cats receivingnormal control diet (p<0.05). Interestingly, the AUCglucoselevelsof HCdietgroup treated with roselle extract at both doses (180.6 ฑ 25.8 and 178.5ฑ10.9 min.mg/dL) were significantly lower than that of HC control group (270.1ฑ19.1min.mg/dL). For 24hoursglucose, the AUCglucose of HC group was significantly (p<0.05) higher than that of normal control diet group, however,treatment with both doses of roselle extract did not affect 24hoursAUC glucose levels of HC fed cats. In addition, the HC diet control group had a significantly higherHOMA-IR score as compared to the normal control diet group. This high HOMA-IR returned to normal level in the group that received roselle extract. In conclusion, theresults indicatethat roselle extract canreduce blood glucoseby reducing insulin resistancein HC fed cats. Roselle calyce extract mighttherefore be used as alternative medicine in type2-diabetic cats. |
