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Effects of polyvinyl alcohol on vitrification of mature and immature bovine oocytes |
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| รหัสดีโอไอ | |
| Creator | Saksiri Sirisathien |
| Title | Effects of polyvinyl alcohol on vitrification of mature and immature bovine oocytes |
| Contributor | ChatreeChumnandee, PinichWangsomnuk |
| Publisher | Research and Technology Transfer Affairs Division.Khon Kaen University. |
| Publication Year | 2560 |
| Journal Title | Asia-Pacific Journal of Science and Technology (APST) |
| Journal Vol. | 22 |
| Journal No. | 4 |
| Page no. | 1-9 |
| Keyword | Bovine oocyte, Cryopreservation, Ice blocking agent, Polyvinyl alcohol, Vitrification |
| URL Website | https://tci-thaijo.org/index.php/APST/index |
| Website title | https://tci-thaijo.org/index.php/APST/article/view/108019 |
| ISSN | 2539-6293 |
| Abstract | The objective of this study was to evaluate the putative effect of low molecular weight (9000-10000 Da) polyvinyl alcohol (PVA) as an ice blocking agent on vitrification of mature (metaphase II stage; MII) and immature (germinal vesicle stage; GV) bovine oocytes. In experiment 1, effect of two concentrations of PVA (0.1% and 1%) on the viability of vitrified-warmed MII oocytes was determined using fluorescein diacetate staining. The results showed that the viability of vitrified-warmed MII oocytes was not significantly differentamong groups (vitrified control; 65.6%, +0.1 %PVA; 83.3%, and+1.0 %PVA; 73.7%). In experiment 2, effect of 0.1% PVA on fertilization and developmental competence of vitrified-warmed MII oocytes was determined. The blastocyst development of oocytes in 0.1%PVA group was significantly higher(p?0.05)than that of vitrified control group(10.1% and 3%, respectively) but significantly lower (p?0.05)than that of fresh control group (59.1%). In experiment 3, the maturation rate of vitrified-warmed GV oocytes was determined. The percentages of vitrified-warmed GV oocytes reaching MII stage were not different among three maturation periods (22h IVM; 33.3%, 24 h IVM; 54.5%, and 26 h IVM; 31.3%). In experiment 4, effect of 0.1% PVA on fertilization and developmental competence of vitrified-warmed GV oocytes was determined. The proportions of blastocysts were not differentbetween vitrified control and 0.1%PVA group (1.5% and 0.8%, respectively). In conclusion, the results showed that a low molecular weight PVA at a concentration of 0.1% can improve vitrification of mature bovine oocytes but lacking positive effect with immature oocytes. |
