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Dioscorea polystachya extract treats menopause and osteoporosis in ovariectomised female mice |
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| รหัสดีโอไอ | |
| Creator | Liyuan Zhang |
| Title | Dioscorea polystachya extract treats menopause and osteoporosis in ovariectomised female mice |
| Contributor | Xiangyi Chen |
| Publisher | Maejo University |
| Publication Year | 2568 |
| Journal Title | Maejo International Journal of Science and Technology |
| Journal Vol. | 19 |
| Journal No. | 3 |
| Page no. | 232 |
| Keyword | Dioscorea polystachya, osteoporosis, osteoblasts, ovariectomy, menopause |
| Website title | Maejo International Journal of Science and Technology |
| ISSN | 1905-7873 |
| Abstract | Pathological osteoporosis (OP) reduces bone strength and density. Menopause is a significant factor contributing to the development of OP. Postmenopausal women lack oestrogen which boosts osteoclastic activity and reduces osteoblast cell activity. This research evaluates the impact of Dioscorea polystachya (DP) aqueous extract on ovarian function in ovariectomised (OVX) female mice. DP (5-40 ?g/mL) was applied to MC3T3-E1 cells for 14 days. The impact on cellular changes was assessed using alkaline phosphatase (ALP) and alizarin red S (ARS) staining. The DP's effects on OP were evaluated in an OVX mouse model. Blood was withdrawn to measure oestradiol and osteocalcin concentrations. A micro-CT scan assessed bone mineral density (BMD), bone microstructure and femur tissue. OVX mouse femurs were histopathologically investigated. The DP group showed considerable improvement in ALP and ARS staining activity compared to the control group. Bone morphogenetic protein 2/Runt-related transcription factor 2 (BMP2/4/Runx2) expression was significantly greater in the DP groups compared to the control group. DP significantly raised serum oestradiol and osteocalcin levels in the tested groups. DP-treated groups expressed higher levels of runt-related transcription factor 2 (Runx2), bone morphogenetic protein 2/4, osteoprotegerin and phosphorylated Smad2/3 than the OVX group. DP enhances the bone tissue architecture and BMD of OVX mice. The study found that DP promotes osteoblasts via the BMP-2/4 and Runx2 pathway in OVX female mice, alleviating menopausal symptoms. |
