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Metagenomic exploration of bacterial community in marine sediment in Gulf of Mannar (Rameshwaram), Tamil Nadu, India |
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| รหัสดีโอไอ | |
| Creator | Kotakonda Muddukrishnaiah |
| Title | Metagenomic exploration of bacterial community in marine sediment in Gulf of Mannar (Rameshwaram), Tamil Nadu, India |
| Contributor | Marappan Makesh, Krishnan Akilandeswari, Jangam Ashok Kumar |
| Publisher | Maejo University |
| Publication Year | 2565 |
| Journal Title | Maejo International Journal of Science and Technology |
| Journal Vol. | 16 |
| Journal No. | 1 |
| Page no. | 1 |
| Keyword | metagenomics, marine sediment, nanopore sequencing, bacterial, community, Gulf of Mannar, India |
| Website title | Maejo International Journal of Science and Technology |
| ISSN | 1905-7873 |
| Abstract | Metagenomics was used to gain a deeper insight into bacterial diversity of the marine soil sediment of the Gulf of Mannar (Ramanathapuram), Tamil Nadu, India. Sequencing was performed using a MinION Oxford Nanopore Sequencer. Fastq read files of the microbial metagenomic data were processed through the MG-RAST server with default parameters. The metagenome contained 10,245 reads with a total of 2,979,291 bp and a G+C content of 51 5 %. In the analysed bacterial community we found 10,245 species in 14 different phyla. The dominant phyla were Proteobacteria (35.62%), Firmicutes (29.18%), Planctomycetes (9.01%), Bacteroidetes (4.29%), Cyanobacteria (3.86%), Actinobacteria (2.58%), Lentisphaerae (2.58%) and Acidobacteria (0.86%). The distribution of phyla suggested a significant anthropogenic influence affecting the diversity of this marine sediment bacterial community. Comparative analysis of genes (rfbB, rmlB and rffG) active in the biosynthesis of secondary metabolites, xenobiotic degradation and metabolism of sulphur, carbon, and nitrogen revealed that the genes involved in heterotrophic processes such as degradation of carbohydrates, hydrocarbons and aromatics were most highly expressed in the marine soil sediment micro-organisms. |
