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Effects of Multi-Strain Microbial Inoculants on Fermentation Quality andIn Vitro Digestibility of Rice Straw Silage |
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| รหัสดีโอไอ | |
| Creator | Jirasak Thongseela |
| Title | Effects of Multi-Strain Microbial Inoculants on Fermentation Quality andIn Vitro Digestibility of Rice Straw Silage |
| Contributor | Choke Sorachakula, Krittanon Chotprom, Supannika Promsuriya, Somchart Tana |
| Publisher | Rajamangala University of Technology Lanna, Thailand |
| Publication Year | 2569 |
| Journal Title | Journal of Science and Agricultural Technology |
| Journal Vol. | 7 |
| Journal No. | 1 |
| Page no. | 8-14 |
| Keyword | Rice straw silage, Multi-strain Inoculants, Lignocellulose degradation, In vitro gas production, Rumenfermentation |
| URL Website | https://jsat.rmutl.ac.th/ |
| Website title | Journal of Science and Agricultural Technology |
| ISSN | 27301524 |
| Abstract | Rice straw is one of the most widely available agricultural by-products in Thailand. However, its directuse as ruminant feed is constrained by low crude protein concentration, high structural fiber content, and thepresence of a lignocellulosic matrix that limits microbial degradation in the rumen. This study evaluated the effectsof selected microbial inoculant combinations on fermentation quality, chemical composition, fiber fractions, and invitro gas production of rice straw silage. Chopped rice straw was assigned to five microbial treatments in acompletely randomized design with three replications: T1, Trichoderma reesei; T2, Saccharomyces cerevisiae; T3,T. reesei + S. cerevisiae; T4, T. reesei + S. cerevisiae + Bacillus licheniformis; and T5, T. reesei + S. cerevisiae +Lactobacillus plantarum. The treated materials were ensiled under anaerobic conditions and evaluated after 7, 14,21, and 28 days of fermentation. Microbial inoculation significantly influenced fiber degradation, particularlyneutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL) (P < 0.001). Among thetreatments, T5 showed the most favorable response after 28 days of fermentation, with the lowest NDF (69.47%),ADF (47.66%), and ADL (19.02%) values. The lowest pH value was also observed in T5 at 21 days (4.65),suggesting improved acidification during ensiling. In vitro gas production differed significantly among treatmentsat 28 days (P < 0.01), with T5 producing the highest gas volume (74.5 mL), indicating greater ruminalfermentability. These results suggest that the combined application of cellulolytic fungi, yeast, and lactic acidbacteria can enhance lignocellulose degradation and improve the feeding value of rice straw silage. Therefore, theinoculant combination of T. reesei + S. cerevisiae + L. plantarum may be considered a promising biological strategyfor improving the utilization of rice straw in ruminant production systems. |
