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Callus Induction and Plantlet Regeneration from Anther of Andrographis paniculata (Burm.f.) Wall.ex Nees. In Vitro |
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| รหัสดีโอไอ | |
| Creator | Therdsak Thonnaluk |
| Title | Callus Induction and Plantlet Regeneration from Anther of Andrographis paniculata (Burm.f.) Wall.ex Nees. In Vitro |
| Contributor | Chantana Witrut, Jinthapa Aum-um |
| Publisher | Faculty of Agriculture, Chiang Mai University |
| Publication Year | 2569 |
| Journal Title | Journal of Agricultural Research and Communications |
| Journal Vol. | 42 |
| Journal No. | 1 |
| Page no. | 15-30 |
| Keyword | King bitter, anther culture, callus, temperature |
| URL Website | https://li01.tci-thaijo.org/index.php/joacmu/article/view/264549 |
| Website title | Journal of Agricultural Research and Communications |
| ISSN | 3057-1022 |
| Abstract | In vitro anther culture of Andrographis paniculata (Burm.f.) Wall.ex Nees. from Chiang Mai for callus induction was studied. The flowers were harvested 9 to 7 days before blooming and were used for anther culture. The anther explants were cultured on Nitsch & Nitsch medium (1967), as modified by Lichter (1982) (NLN), supplemented with 0, 0.1, 0.5, and 1.0 mg/L 6-Benzylaminopurine (BAP) in combination with 0, 0.1, and 0.5 mg/L Naphthalene acetic acid (NAA) for 8 weeks. The results showed that the most effective treatment for callus induction was cultured on NLN medium supplemented with 0.5 mg/L NAA in combination with 1.0 mg/L BAP. In order to increase the callus and induce embryo, callus was cultured at the low temperature of 4?C for 1 and 3 days, it was further transplanted under high temperature of 28, 30 and 32?C for 1, 2, 3 and 4 days on NLN medium supplemented with 0.5 mg/L NAA in combination with 1.0 mg/L BAP. The results showed that the highest weight callus and induced somatic embryo were cultured at a low temperature of 4?C for 1 day and then transplanted at a high temperature of 28?C for 1 day could increase the quantity of callus and induce to form an embryo. |
