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Evaluation of cholesterol-related functional traits of Bacillus infantis 63-11 under in vitro simulated small intestinal conditions and genomic analysis |
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| รหัสดีโอไอ | |
| Creator | Suwapat Saejie |
| Title | Evaluation of cholesterol-related functional traits of Bacillus infantis 63-11 under in vitro simulated small intestinal conditions and genomic analysis |
| Contributor | Varapha Kongpensook, Nattida Chotechuang, Cheunjit Prakitchaiwattana |
| Publisher | Mahasarakham University |
| Publication Year | 2569 |
| Journal Title | Food Agricultural Sciences and Technology (FAST) |
| Journal Vol. | 12 |
| Journal No. | 3 |
| Page no. | 255-273 |
| Keyword | Bacillus infantis, Bile salt hydrolase, Cholesterol reduction, Whole-genome sequencing |
| URL Website | https://ph02.tci-thaijo.org/index.php/stej/index |
| Website title | Food Agricultural Sciences and Technology (FAST) |
| ISSN | 2822-1001 (Print), 2822-101X (Online) |
| Abstract | Bacillus infantis 63-11 is a microorganism isolated from traditional Thai fermented fish and shows potential for probiotic development. The study evaluated cholesterol-related functional traits alongside genomic characterization and bile salt hydrolase activity. Species identification through 16S rRNA gene sequencing showed 98.13-99.35% similarity to reference strains. Whole-genome analysis yielded an ANIb of 96.34% and dDDH value of 86.0%, both exceeding species identification thresholds. The TDCA halo assay demonstrated bile salt hydrolase activity with precipitation halos averaging 1.88 +/- 0.48 mm in diameter. In vitro cultivation resulted in a 78.21% cholesterol reduction after 48 hours. Under simulated intestinal conditions with 0.3% bile salts, strain 63-11 persisted in both vegetative and spore forms. The findings suggest BSH-related processes may contribute to the observed phenotype, though additional in vivo studies are needed for confirmation. |
