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Morphological and physiological responses of ornamental banana (Musa ornata Roxb.) to sodium azide mutagenesis |
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| รหัสดีโอไอ | |
| Creator | Nattapong Srisamoot |
| Title | Morphological and physiological responses of ornamental banana (Musa ornata Roxb.) to sodium azide mutagenesis |
| Publisher | Mahasarakham University |
| Publication Year | 2569 |
| Journal Title | Food Agricultural Sciences and Technology (FAST) |
| Journal Vol. | 12 |
| Journal No. | 2 |
| Page no. | 155-167 |
| Keyword | Musa ornata, sodium azide, tissue culture, induced mutation, chlorophyll content |
| URL Website | https://ph02.tci-thaijo.org/index.php/stej/index |
| Website title | Food Agricultural Sciences and Technology (FAST) |
| ISSN | 2822-1001 (Print), 2822-101X (Online) |
| Abstract | Induced mutagenesis represents a practical approach for generating genetic variability in ornamental bananas, where conventional breeding is constrained by sterility and parthenocarpy. This study evaluated the morphological and physiological responses of Musa ornata Roxb. plantlets to sodium azide (NaN3) treatment under in vitro conditions. Uniform plantlets were exposed to NaN3 concentrations ranging from 0.0 to 4.0 mM for 1 h, and survival rate, growth-related traits, photosynthetic pigment contents, and leaf color parameters were assessed after 16 weeks of culture. Survival rate declined in a clear concentration-dependent manner, allowing the estimation of LD30 and LD50 values at 1.13 and 2.08 mM, respectively. Among the evaluated traits, fresh weight showed a statistically significant increase at a low NaN3 concentration (0.1 mM), whereas leaf number, pseudostem length, root traits, photosynthetic pigments, and leaf color parameters exhibited only numerical variation without consistent statistical significance. Chlorophyll and carotenoid contents, as well as CIELAB leaf color parameters, did not differ significantly among treatments. These results indicate that NaN3 primarily affects M. ornata plantlets through concentration-dependent survival responses, while most morphological and physiological traits display descriptive variation at the in vitro stage. The study provides species-specific lethal dose reference values and baseline phenotypic screening information for the application of NaN3 in ornamental banana mutation breeding. Further studies are required to determine the stability and breeding value of NaN3-induced variations after the early screening stage. |
