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UV-Vis Spectrophotometric Method Using Natural Reagent from Vigna unguiculata subsp.sesquipedalisfor Tetracycline Determination in Pharmaceutical Samples |
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| รหัสดีโอไอ | |
| Creator | Kanyarak Prasertboonyai |
| Title | UV-Vis Spectrophotometric Method Using Natural Reagent from Vigna unguiculata subsp.sesquipedalisfor Tetracycline Determination in Pharmaceutical Samples |
| Contributor | Senee Kruanetr |
| Publisher | King Mongkut's Institute of Technology Ladkrabang |
| Publication Year | 2564 |
| Journal Title | Current Applied Science and Technology |
| Journal Vol. | 21 |
| Journal No. | 1 |
| Page no. | 51-64 |
| Keyword | Vigna unguiculatasubsp. sesquipedalis, tetracycline, UV-Visspectrophotometer |
| URL Website | https://www.tci-thaijo.org/index.php/cast |
| Website title | https://www.tci-thaijo.org/index.php |
| ISSN | 2586-9396 |
| Abstract | The natural reagents from Vigna unguiculatasubsp.sesquipedaliswere studied and applied in the determination of tetracycline by UV-Vis spectrophotometry. The method was based on a complexation formation between tetracycline and iron(III)derived from natural plant extract in acetate buffer at pH 5to give a yellow complex with the optimumabsorption at 430 nm. Parameters related to the extraction efficiency of the natural reagent and the factors that affected the determination of tetracycline were examined. Under optimum conditions, linearity was obtained over the range of 1.00 -20.00 mg l-1. The limit of detection (LOD, 3?) and limit of quantification (LOQ, 10?), calculated following IUPAC, were 0.65 and 2.15 mg l-1, respectively. The repeatability and reproducibility for determining 10.00 mg l-1of tetracycline (n=11)were 3.43%and 5.14%, respectively. The proposed method was successfully applied to the determination of tetracycline in pharmaceutical formulations. The results obtained by the proposed method were in good agreement with the label values verified by the student t-test at the 95%confidence level. |
