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Micropropagation of Koelreuteria bipinnata Using Juvenile and Mature Explants |
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| รหัสดีโอไอ | |
| Creator | Azza A. Tawfik |
| Title | Micropropagation of Koelreuteria bipinnata Using Juvenile and Mature Explants |
| Contributor | Omer H. Ibrahim and Mona A. Taha |
| Publisher | King Mongkut's Institute of Technology Ladkrabang |
| Publication Year | 2563 |
| Journal Title | Current Applied Science and Technology |
| Journal Vol. | 20 |
| Journal No. | 3 |
| Page no. | 470-478 |
| Keyword | BAP, Chinese flame tree, Juvenile nodal explants, Mature nodal explants, Sucrose, Woody plants |
| URL Website | https://www.tci-thaijo.org/index.php/cast |
| Website title | https://www.tci-thaijo.org/index.php |
| ISSN | 2586-9396 |
| Abstract | An efficient micropropagation protocol for Koelreuteria bipinnata, an ornamental tree, from mature and juvenile phase tissues was described. Nodal explants from one-year-old branches of a field-grown mature tree (mature phase) and from in vitro growing seedlings (juvenile phase) were used. The nodal explants from mature explants cultured on Murashig& Skoog (MS) medium supplemented with or without growth regulators did not show any axillary shoot development. However, 6-benzylaminopurine (BAP) at 1.5 mg/l induced axillary shoots from all juvenile explants (100%). It also produced the highest number of shoots (2.5 shoots per explant), the highest rooting percentage (100%), the maximum number of roots (2.80 roots/shoot) and the longest roots (5.87 cm) when it was added as a supplement to the half-strength MS medium culture that included 15 g/l sucrose. Plantlets were successfully acclimatized and transferred to the field with 60% survival rate. |
