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High methane production by Methanobacterium ferruginis Mic6c05T, a hydrogenotrophic methanogen isolated from a depleted oil and gas field in a neighboring country. |
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| รหัสดีโอไอ | |
| Creator | Siriwan Wichai |
| Title | High methane production by Methanobacterium ferruginis Mic6c05T, a hydrogenotrophic methanogen isolated from a depleted oil and gas field in a neighboring country. |
| Contributor | Nusara Yinyom, Kusuma Rintachai |
| Publisher | Khon Kaen University, Thailand |
| Publication Year | 2568 |
| Journal Title | Asia-Pacific Journal of Science and Technology |
| Journal Vol. | 30 |
| Journal No. | 6 |
| Page no. | 10 (13 pages) |
| Keyword | methanogenesis, archaea, biogenic gas, methanogenic media, phylogenetic analysis, MPN |
| URL Website | https://apst.kku.ac.th/ |
| Website title | https://apst.kku.ac.th/high-methane-production-by-methanobacterium-ferruginis-mic6c05t-a-hydrogenotrophic-methanogen-isolated-from-a-depleted-oil-and-gas-field-in-a-neighboring-country/ |
| ISSN | 2539-6293 |
| Abstract | Some depleted oil and gas fields harbor anaerobic microbes, including methanogens. In this study, methanogens were isolated from a depleted oil and gas field in a neighboring country using enrichment techniques in methanogenic media (ATCC 1340). Five isolates were obtained. Among them, three isolates- ATCC 06.2-1, ATCC 06.2-2, and ATCC reactor 1- demonstrated a strong ability to produce high levels of methane in the ATCC 1340 medium. This culture medium is specifically designed to convert H2 and CO2 at a ratio of 4:1, leading to a theoretical total yield of 249.82 µmol of methane. The results indicated that ATCC 06.2-1, ATCC 06.2-2, and ATCC reactor 1 achieved methane production percentages of 94.07%, 75.65%, and 72.05% of the theoretical yield, respectively. Additionally, all three isolates could utilize formate and methanol as substrates for methane production and were classified as hydrogenotrophs. Genetic identification revealed that the three methane-producing isolates- ATCC 06.2-1, ATCC 06.2-2, and ATCC reactor 1- belong to the species Methanobacterium ferruginis Mic6c05T. The other two isolates, ATCC 03 and ATCC 04.2, were identified as Clostridium sporogenes DSM 795T. The three methane-producing isolates and the two Clostridium isolates show significant potential for optimizing alternative energy sources. |
