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In vitro anti-leukemic activity of Terminalia arjuna bark extract loaded chitosan nanoparticles on HL-60 cell lines |
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| รหัสดีโอไอ | |
| Creator | Natesan Geetha |
| Title | In vitro anti-leukemic activity of Terminalia arjuna bark extract loaded chitosan nanoparticles on HL-60 cell lines |
| Contributor | Gokul Murukadas, Anju Rani George, Hansiya V S, Sradha Sajeev, Kavimani Thangasamy, Aarthi Jeganathan, Anju Byju |
| Publisher | Khon Kaen University, Thailand |
| Publication Year | 2568 |
| Journal Title | Asia-Pacific Journal of Science and Technology |
| Journal Vol. | 30 |
| Journal No. | 6 |
| Page no. | 3 (12 pages) |
| Keyword | Chitosan nanoparticles, Terminalia arjuna, Cytotoxicity, HL-60 cell lines, In vitro drug release kinetics, Leukemia cancer |
| URL Website | https://apst.kku.ac.th/ |
| Website title | https://apst.kku.ac.th/in-vitro-anti-leukemic-activity-of-terminalia-arjuna-bark-extract-loaded-chitosan-nanoparticles-on-hl-60-cell-lines/ |
| ISSN | 2539-6293 |
| Abstract | The bark of Terminalia arjuna (Roxb. ex DC.) Wight & Arn. has been traditionally used in Indian medicine for treating various ailments, including leukemia. Chitosan, a natural biopolymer, serves as a promising carrier for targeted drug delivery. This study focuses on the encapsulation of hexane-ethanolic bark extract (HEB) of T. arjuna using chitosan nanoparticles (CSNPs) and evaluates their anti-leukemic potential against HL-60 cell lines. The HEB-loaded CSNPs (HEB-CSNPs) were synthesized and their entrapment efficiency, drug release profile and cytotoxic activity were assessed. In vitro release studies demonstrated a controlled and sustained drug release of 96.66% within three hours. Drug release kinetics followed the Higuchi model with a correlation coefficient (R²) of 0.98, indicating diffusion-controlled release. Fourier transform infrared (FTIR) spectra confirmed the presence of characteristic functional groups of both chitosan and the plant extract. X-ray diffractometer (XRD) analysis revealed an increase in nanoparticle size from 56.88 nm (control) to 64.53 nm upon encapsulation. Search engine marketing (SEM) imaging showed well-dispersed nanoparticles with a large surface area. Cytotoxicity analysis on HL-60 cells demonstrated dose-dependent activity, with an inhibitory concentration (IC) IC50 value of 185.2 μg/mL for HEB-CSNPs. Conclusion: Overall, this study demonstrates that CSNPs are effective carriers for T. arjuna bark extract, ensuring efficient encapsulation, sustained release, and significant anti-leukemic activity in vitro. These findings suggest the potential of HEB-CSNPs as a biocompatible and eco-friendly nanomedicine for leukemia treatment and warrant further investigation in preclinical models. |
