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Study on kinship among kepok banana cultivars using resistance gene analogue (RGA) based single nucleotide polymorphism (SNP) markers |
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| รหัสดีโอไอ | |
| Creator | Rochmah Agustrina |
| Title | Study on kinship among kepok banana cultivars using resistance gene analogue (RGA) based single nucleotide polymorphism (SNP) markers |
| Contributor | Eti Ernawiati, Wasinton Simanjuntak, Bambang Irawan, Sutopo Hadi |
| Publisher | Asia-Pacific Journal of Science and Technology |
| Publication Year | 2567 |
| Journal Title | Asia-Pacific Journal of Science and Technology |
| Journal Vol. | 29 |
| Journal No. | 5 |
| Page no. | 16 (8 pages) |
| Keyword | Kepok banana, single nucleotide polymorphism, resistance gene analog, phylo-genetic three |
| URL Website | https://so01.tci-thaijo.org/index.php/APST/ |
| Website title | https://so01.tci-thaijo.org/index.php/APST/article/view/268442 |
| ISSN | 2539-6293 |
| Abstract | The primary goal of the research is to implement Resistance Gene Analog (RGA) markers to determine the kinship of three kepok banana cultivars from Bandarlampung: kepok abu (AAB genome), kepok batu (ABB genome), and kepok kuning (BBB genome). The banana RGA genome sequence from GenBank (ID: KF034945 to KF034953) was used to examine the sequencing data for parallels. Phylogenetic trees were constructed with 1000 bootstrap replicates of neighbor-joining using MEGA X. It was determined that the primer SNP2-MNBS-Ref. may identify Single Nucleotide Polymorphism (SNP) sites at the 327th nucleotide base pair, which is the cytosine nucleotide base, in the RGA of the three banana cultivars that have been validated in GenBank. The phylogenetic tree showed that the three banana cultivars have a common ancestor, which is Musa balbisiana cv. Klutuk Wulung. With a genetic distance of 29%, the progenitor created a branch where Kepok Batu and Kepok Kuning are in the same clade as Musa acuminata subsp. burmannica cv. Calcuta4. Kepok Abu is in the same clade as Musa acuminata cv. Rejang and Musa acuminata subsp. Malaccensis, with a genetic distance of 28%. This indicates that Kepok Batu and Kepok Kuning are one cultivar but distinct variations. Consequently, it may be said that each of the three banana cultivars studied has an SNP location in the R gene. Kepok Abu's reduced genetic distance indicates that it may be a good source of R gene material for the Musa improvement program, which aims to produce a cultivar with increased pathogen resistance. |
