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Validation and application of HPLC method for determination of cordycepin and adenosine in dietary supplements |
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| รหัสดีโอไอ | |
| Creator | Pattana Sripalakit |
| Title | Validation and application of HPLC method for determination of cordycepin and adenosine in dietary supplements |
| Contributor | Naruemon Chernthong, Suphisara Noisakul, Aurasorn Saraphanchotiwitthaya |
| Publisher | Asia-Pacific Journal of Science and Technology |
| Publication Year | 2565 |
| Journal Title | Asia-Pacific Journal of Science and Technology |
| Journal Vol. | 27 |
| Journal No. | 6 |
| Page no. | 9 |
| Keyword | Adenosine, Cordycepin, Cordyceps, Dietary supplements, HPLC-UV |
| URL Website | https://www.tci-thaijo.org/index.php/APST |
| Website title | https://so01.tci-thaijo.org/index.php/APST/article/view/262655 |
| ISSN | 2539-6293 |
| Abstract | A simple method based on High Performance Liquid Chromatography (HPLC)-UV was developed in this study for determining the existence of cordycepin and adenosine in Cordyceps and its products. Chromatographic separation was carried out using a Kinetex? C18 column with a mobile phase of water and acetonitrile (95:5, v/v), a flow rate of 1.0 mL/min, and wavelengths of ultraviolet detection set at 260 nm. The calibration curve was linear over the range of 0.0520 and 0.0220 ?g/mL for cordycepin and adenosine, respectively, and the limit of detection was 0.005 ?g/mL for both compounds. The method was validated with excellent specificity, accuracy, precision, and recovery. The assay has been applied successfully to a quality control study on commercial products containing Cordyceps in the Thai market such as dried cultured materials and pharmaceutical formulations. |
