Effect of cell density and nutrient deprivation on hydrogen production by unicellular green algaScenedesmus sp. KMITL-OVG1
รหัสดีโอไอ
Creator Saranya Phunpruch
Title Effect of cell density and nutrient deprivation on hydrogen production by unicellular green algaScenedesmus sp. KMITL-OVG1
Contributor Kittiphat Warichanan
Publisher Asia-Pacific Journal of Science and Technology
Publication Year 2562
Journal Title Asia-Pacific Journal of Science and Technology
Journal Vol. 24
Journal No. 2
Page no. 1-9
Keyword hydrogen production, nutrient deprivation, green algae, Scenedesmus sp.
URL Website https://www.tci-thaijo.org/index.php/APST
Website title https://www.tci-thaijo.org/index.php/APST/article/view/114922
ISSN 2539-6293
Abstract Hydrogen is considered as one of the energy carriers for the near future. H2production by green algae is catalyzed by hydrogenase activity using electrons from photosynthetic process under the light and from accumulated carbohydrate catabolism in the dark. This research aimed to investigate the effect of cell density and nutrient deprivation on H2production by Scenedesmus sp. KMITL-OVG1 isolated in Thailand. The result showed that cell culture with the optical density at 750 nm of 0.8 gave the highest H2production rate. Interestingly, the highest H2production rateof 1.957ฑ 0.100 mL L-1h-1and hydrogenase activity of 0.031ฑ 0.001 ml L-1min-1werefound in cells incubated under potassium deprivation. H2production rate was approximately 3 foldshigher than that of cells incubated in normal TAP medium. TheincreasedH2production rate and hydrogenase activity might be involved in the reduction of starch accumulation. Moreover, the deprivation of potassium combined with other nutrients did not enhance H2production rate by Scenedesmus sp. KMITL-OVG1.
Asia-Pacific Journal of Science and Technology

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