Polyhydroxyalkanoate production from palm oil factory wastes and its application for 3-hydroxyalkanoate methyl esters as biofuels
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Title Polyhydroxyalkanoate production from palm oil factory wastes and its application for 3-hydroxyalkanoate methyl esters as biofuels
Creator 1. Pipat Junpadit
2. Piyarat Boonsawang
3. Thunwadee Tachapattaworakul Suksaroj
Publisher Joint Graduate School of Energy and Environment
Publication Year 2557
Keyword 3-hydroxyalkanoate methyl esters, 3HAME, polyhydroxyalkanoate, PHA, PHA accumulating bacteria, biodiesel
Abstract Polyhydroxyalkanoate (PHA) synthesized and stored in the various microorganism cell cytoplasms. In this study, the palm oil factory wastes; liquid after squeezing the empty fruit branch (LEFB), palm oil mill effluent (POME) and biogas tank effluent (BTE), was used as substrate for PHA production. The result showed that POME gave the highest dry cell weight (DCW) of 2.97 g/L and PHA production of 2.27 g/L. Then POME was selected for the large amount of PHA production in 5 L aeration reactor and then acid hydrolysis for 3-Hydroxyalkanoate methyl esters (3HAME) production. The 44.4% of 3HAME content was obtained. The properties of 3HAME has met the regulation standard of ASTM D6751-07 with a heating value, methyl ester content, flash point and pour point of 32.9 KJ/g, 96.8%, 132?C and 7?C, respectively. Therefore, the 3HAME from extracted PHA using POME as a sole carbon source has a feasibility to be an alternative biodiesel fuel.
ISBN 978-616-92228-1-1
Joint Graduate School of Energy and Environment

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